High Quality Standard cDNA Libraries are produced using our proprietary technology. First strand cDNA is made from mRNA that is primed using oligo dT. After the second strand is synthesized, the double stranded cDNA is size fractionated, cloned directionally into our Express I vector and transformed into T1 phage resistant E. coli. We guarantee at least 3×106 primary clones with ≥87% recombinant clones containing an average insert size of at least 1kb, however, we generally produce libraries of 10×106 primary clones with >95% recombinant clones containing ≥1.5kb average insert size.
We will make standard libraries from customer supplied tissue (≥1 g), cells (at least 1×108), total RNA (≥1 mg) or mRNA (≥5 µg). We provide quality controlled primary or amplified libraries.
Characteristics of a cDNA Library Constructed From a Standard Amount of HeLa mRNA
|mRNA (ng)||Total # of primary clones||Average insert size (kb)*||Percent recombinants||Insert size range (kb)*|
|5,000||12.0×107||2.24||96||0.5 – 4.4|
mRNA (ng), Total # of primary clones, Average insert size (kb)*, Percent recombinants, Insert size range (kb)**Average insert size and insert size range determined by restriction enzyme digestion of 24 clones picked at random from each library.
Quality testing: Percentage of recombinant clones and average insert size determined by gel analysis of 24 clones picked at random.
Shipment and storage conditions: Libraries will be shipped in dry ice. Storage of libraries at -80°C is recommended.