High Quality Large Insert cDNA Libraries are constructed using our state-of-the-art methods for cDNA synthesis and size fractionation to produce libraries containing the largest cDNA inserts available. Although these libraries are made using a different process, they share the same advantageous features as our standard libraries (directional cloning into Express I and T1 phage resistant E. coli). We guarantee at least 1×106 primary clones with ≥87% recombinant clones containing an average insert size of at least 3kb, however, we generally produce libraries of 3×106 primary clones with >95% recombinant clones containing ≥4kb average insert size.
We will make large insert libraries from customer supplied tissue (≥2 g), cells (at least 2×108), total RNA (≥2 mg) or mRNA (≥10 µg). We provide quality controlled primary or amplified libraries.
Characteristics of a cDNA Library Constructed From Large Transcripts of HeLa mRNA
|mRNA (ng)||Total # of primary clones||Average insert size (kb)*||Percent recombinants||Insert size range (kb)*|
|5,000||2.1×107||4.26||96||1.0 – 6.2|
|2,000||2.5×106||4.46||100||2.4 – 7.2|
mRNA (ng), Total # of primary clones, Average insert size (kb)*, Percent recombinants, Insert size range (kb)**Average insert size and insert size range determined by restriction enzyme digestion of 24 clones picked at random from each library.
Quality testing: Percentage of recombinant clones and average insert size determined by gel analysis of 24 clones picked at random.
Shipment and storage conditions: Libraries will be shipped in dry ice. Storage of libraries at -80°C is recommended.